6B00
Thermostabilized mutant of human carbonic anhydrase II - A65T L100H K154N L224S L240P A248T
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Carbonic anhydrase 2 | polymer | 266 | 30129.8 | 1 | UniProt (P00918) Pfam (PF00194) | Homo sapiens (Human) | Carbonate dehydratase II,Carbonic anhydrase C,CAC,Carbonic anhydrase II,CA-II |
| 2 | B (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 3 | C (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 4 | D (A) | water | water | 18.0 | 409 | Chemie (HOH) |
Sequence modifications
A: 3 - 261 (UniProt: P00918)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Ala 2 | - | expression tag |
| Thr 65 | Ala 65 | engineered mutation |
| His 100 | Leu 100 | engineered mutation |
| Asn 154 | Lys 153 | engineered mutation |
| Ser 224 | Leu 223 | engineered mutation |
| Pro 240 | Leu 239 | engineered mutation |
| Thr 248 | Ala 247 | engineered mutation |
| His 262 | - | expression tag |
| His 263 | - | expression tag |
| His 264 | - | expression tag |
| His 265 | - | expression tag |
| His 266 | - | expression tag |
| His 267 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 30129.8 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 157.5 | |
| All* | Total formula weight | 30287.3 |
