1A0P
SITE-SPECIFIC RECOMBINASE, XERD
Summary for 1A0P
| Entry DOI | 10.2210/pdb1a0p/pdb |
| Descriptor | SITE-SPECIFIC RECOMBINASE XERD (2 entities in total) |
| Functional Keywords | xerd, recombinase, dna binding, dna recombination |
| Biological source | Escherichia coli |
| Cellular location | Cytoplasm (By similarity): P0A8P8 |
| Total number of polymer chains | 1 |
| Total formula weight | 33308.19 |
| Authors | Subramanya, H.S.,Arciszewska, L.K.,Baker, R.A.,Bird, L.E.,Sherratt, D.J.,Wigley, D.B. (deposition date: 1997-12-05, release date: 1998-03-18, Last modification date: 2024-02-07) |
| Primary citation | Subramanya, H.S.,Arciszewska, L.K.,Baker, R.A.,Bird, L.E.,Sherratt, D.J.,Wigley, D.B. Crystal structure of the site-specific recombinase, XerD. EMBO J., 16:5178-5187, 1997 Cited by PubMed Abstract: The structure of the site-specific recombinase, XerD, that functions in circular chromosome separation, has been solved at 2.5 A resolution and reveals that the protein comprises two domains. The C-terminal domain contains two conserved sequence motifs that are located in similar positions in the structures of XerD, lambda and HP1 integrases. However, the extreme C-terminal regions of the three proteins, containing the active site tyrosine, are very different. In XerD, the arrangement of active site residues supports a cis cleavage mechanism. Biochemical evidence for DNA bending is encompassed in a model that accommodates extensive biochemical and genetic data, and in which the DNA is wrapped around an alpha-helix in a manner similar to that observed for CAP complexed with DNA. PubMed: 9311978DOI: 10.1093/emboj/16.17.5178 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.5 Å) |
Structure validation
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