3EWS
Human DEAD-box RNA-helicase DDX19 in complex with ADP
Summary for 3EWS
| Entry DOI | 10.2210/pdb3ews/pdb |
| Descriptor | ATP-dependent RNA helicase DDX19B, ADENOSINE-5'-DIPHOSPHATE (3 entities in total) |
| Functional Keywords | rna helicase, dead, adp, structural genomics, structural genomics consortium, sgc, rrna, atp-binding, hydrolase, nucleotide-binding, rna-binding, mrna, alternative splicing, cytoplasm, helicase, membrane, mrna transport, nuclear pore complex, nucleus, protein transport, translocation, transport |
| Biological source | Homo sapiens (Human) |
| Cellular location | Cytoplasm: Q9UMR2 |
| Total number of polymer chains | 2 |
| Total formula weight | 101848.22 |
| Authors | Lehtio, l.,Karlberg, t.,Andersson, j.,Arrowsmith, c.h.,Berglund, h.,Bountra, c.,Collins, r.,Dahlgren, l.g.,Edwards, a.m.,Flodin, s.,Flores, a.,Graslund, s.,Hammarstrom, m.,Johansson, a.,Johansson, i.,Kotenyova, t.,Moche, m.,Nilsson, m.e.,Nordlund, p.,Nyman, t.,Olesen, k.,Persson, c.,Sagemark, j.,Thorsell, a.g.,Tresaugues, l.,Van den berg, s.,Weigelt, j.,Welin, m.,Wikstrom, m.,Wisniewska, m.,Schueler, h.,Structural Genomics Consortium (SGC) (deposition date: 2008-10-16, release date: 2008-11-04, Last modification date: 2023-12-27) |
| Primary citation | Collins, R.,Karlberg, T.,Lehtio, L.,Schutz, P.,van den Berg, S.,Dahlgren, L.G.,Hammarstrom, M.,Weigelt, J.,Schuler, H. The DEXD/H-box RNA Helicase DDX19 Is Regulated by an {alpha}-Helical Switch. J.Biol.Chem., 284:10296-10300, 2009 Cited by PubMed Abstract: DEXD/H-box RNA helicases couple ATP hydrolysis to RNA remodeling by an unknown mechanism. We used x-ray crystallography and biochemical analysis of the human DEXD/H-box protein DDX19 to investigate its regulatory mechanism. The crystal structures of DDX19, in its RNA-bound prehydrolysis and free posthydrolysis state, reveal an alpha-helix that inserts between the conserved domains of the free protein to negatively regulate ATPase activity. This finding was corroborated by biochemical data that confirm an autoregulatory function of the N-terminal region of the protein. This is the first study describing crystal structures of a DEXD/H-box protein in its open and closed cleft conformations. PubMed: 19244245DOI: 10.1074/jbc.C900018200 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.7 Å) |
Structure validation
Download full validation report
